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12-Nov-2019 11:05

Higher [Ca2 ]i increased Ca M binding especially in the nucleus and at Z-lines and significantly slowed F-Ca M dissociation rate when F-Ca M was washed out of permeabilized myocytes.Additionally, in both permeabilized and intact myocytes, Ca M moved into the nucleus when [Ca2 ]i was elevated, and this was reversible.■ Reference Data ■ NORMAL : Max Power=8.7 (ps) Max Torque=10.4 (N-M) Cam free : Max Power=11.2 (ps) Max Torque=15.3 (N-M) * Differ from actual driving KITACO measurement value per. * I-map injection controller Ver.2 (763-1432010) must be used.Sold separately * KITACO GPR stainless-steel up exhaust (548-1432100) is recommended.Using 100 n M [Ca M]free we washed F-Ca M into permeabilized myocytes and saw a two-phase (fast and slow) Ca M binding curve with a plateau after 40 min of F-Ca M wash-in.

The presence of Mg2 affects the interaction between dansyl-Ca M and the gamma subunit, as indicated by the increase in the polarization of fluorescence of dansyl-Ca M.While much of Ca M is thought to be protein bound, quantitative data in cardiac myocytes is lacking regarding Ca M location, [Ca M]free and Ca M redistribution during changes in [Ca2 ]i.



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